Stephen's Harvest

Petri dishes containing M. smegmatis bacteria are shown below.  The spots are where phage have destroyed (lysed) bacteria.  We also have electron-micrographs (pictures at 50,000 magnification) and DNA analysis of these phage.  We would have posted these online, but we have found out that the scientific publication copyright on certain scientific journals (PDF file, search for the word "web" within) restricts us from this.

NR2-03-17 F1                                        BG8-03-8 A F1

     

BG8-03-8 B F1                                    NR2-03-17 F2*

   

 

NR2-03-17 F1 and F2, respectively.    BG5-03-5 F2.

  

 

F1 morphologies were witnessed to be the same at F2 unless otherwise noted herein. 

NR2-03-17 F1 is at the 10-7 dilution as picked from a single plaque.  BG8-03-8 A F1 is picked from a plaque diluted to 10-5.  BG8-03-8 B F1 is at the 10-1 dilution, as picked from a single plaque.  It is important to note that the plaque morphology of BG8-03-8 B is the result, in part, of a lower concentration of M. smegmatis when top-agar plated.  The NR2-03-17 F2 * plate is F2 of the NR2-03-17 F1 phage, however the growth time is 1 day less and the phage has not yet formed any turbidity. 

Note that NR2-03-17 F1, comparing to NR2-03-17 F2 have different plaque morphologies, with the F1 having an enlarged outer turbid ring.  This is the result of the duration of growth.  The F1 had grown at 37C for 5 days and 2 days at room temp., while the F2 had grown at 37C for 4 days.  After the F2 was grown for additional time, it also obtained the phenotype of the F1. 

BG5-03-5 F2 is a phage that produces small plaques and tends to have a small number of phage per plaque, when plaques are diluted (comparatively).  The pattern shown has been repeated several times, this plate has held this particular plaque morphology for 2 weeks at 37C.