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Petri dishes containing M. smegmatis bacteria are shown below.  The spots are where phage have destroyed (lysed) bacteria.  We also have electron-micrographs (pictures at 50,000 magnification) and DNA analysis of these phage.  We would have posted these online, but we have found out that the scientific publication copyright on certain scientific journals (PDF file, search for the word "web" within) restricts us from this.

BG3-03-3 A F1                            BG3-03-3 C F1

       

BG3-03-3 B F1 10-1                          BG3-03-3 B F1 10-3

          

 

BG3-03-3 A has been purified, and repurified further than the above.  It still has contamination with a phage that has a morphology as that of the BG3-03-3 C phage.  BG3-03-3 C F1 has been purified through the F2 generation with the same plaque morphology. 

BG3-03-3 B appears to have two different morphologies, these differences are a function of the phage concentration.  The two BG3-03-3 B F1 plates above were picked from the same plaque, and plated on identical smeg.  The phenotype changes as perhaps the result of neighboring plaques, which seem to create a different plaque morphology as a result of .  In the F2, separate plaques were picked, and both phages generated the same dilution patterns shown above.

BG3-03-3B appears to express two different phenotypes depending on the concentration of the phage.  When the concentration is high and the phage is more crowded it forms medium sized clear plaques.  As the phage becomes more dilute the larger plaques wane in numbers as smaller turbid plaques appear in large numbers.  As the dilution progresses the plaque morphology of the phage makes the complete shift from clear to turbid.