Genevieve's Revelations

Petri dishes containing M. smegmatis bacteria are shown below.  The spots are where phage have destroyed (lysed) bacteria.  We also have electron-micrographs (pictures at 50,000 magnification) and DNA analysis of these phage.  We would have posted these online, but we have found out that the scientific publication copyright on certain scientific journals (PDF file, search for the word "web" within) restricts us from this.

DFG-03-33 F1                                   CP1-03-42 A  F1

 

CP1-03-42  F0                                    DFG-03-33-F2

 

DFG-03-33 F1 is the 10-4 dilution of DFG-03-33 plaques.  The plaque morphology of CP1-03-42 A, although only one phage, is not always a dark bulls-eye.   This phage eventually develops a larger, more turbid plaque that has a "dark-halo."  CP1-03-42 F0 had 2 different plaque morphologies, indicating 2 different phages, A and B.  The B phage is (Lazlo continued studies with this phage) the smaller plaque on the F0 plate, notice that the A phage has developed into a larger plaque morphology than in the picture above, as a result of longer incubation time (10 days at 37C) compared to the other phage; which spent a day at 37 and another at RT before these photographs. 

Notice the DFG-03-33-F2 has 3 different plaque morphologies despite having been isolated from plaques 2 additional times (see photo above).  This plate was grown at 37C for 2 days.